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Immunofluorescence (IF) using (a) the unmodified primary rat anti-human CD44 antibody and a secondary donkey anti-rat IgG Alexa Fluor 488 antibody on A549 and HepG2 cells, (b) dual DBCO/Atto488-labelled CD44 antibodies in A549 cells and (c) free NHS ester-Atto488 molecules. Scale bar is 10 µm.
