Figure 5.

Intracellular localization of RhoB-loaded αCD44-PEI-MSNPs (green) with respect to the lysosomes (Lysotracker Deep Red, magenta) over time. A549 cells were incubated with αCD44-PEI-MSNPs (final concentration of 50 μg/mL) for (a) 24 h (b) 48 h and (c) 72 h. The first column shows a complete merge including the transmission image, also displaying the cell areas. In the second and third columns, the lysosomes and nanoparticles are depicted, in purple and green, respectively. In the fourth and fifth columns, a channel overlay and respective magnification are shown. Scale bar is 10 μm in the main images and 3 μm in the magnified images.