Figure 2.

The effect of food restriction on the levels of glucocorticoid and mineralocorticoid receptors in the rat cortex during aging. (A) Representative blot with full-length glucocorticoid receptor protein and shorter GRα protein isoforms (molecular weights indicated). Relative protein levels of the full-length glucocorticoid receptor (B), 70 kDa, 40 kDa, and 25 kDa GR isoforms additionally normalized to the values obtained in control (Ctrl.), 6-month-old animals presented as the line (C), GR mRNA (D), mineralocorticoid receptor (MR) mRNA (E), and GR phosphorylated at Ser²³² (pGR) (F) were determined in rats fed ad libitum (AL) (gray bars) or subjected to food restriction (FR) (black bars). (G) The level of GR phosphorylated at Ser²³² relative to the level of total GR. Data are presented as the mean ± SEM of fold changes relative to the values obtained in control, 6-month-old AL animals (n = 6). * p < 0.05 vs. 6-month-old rats; # p < 0.05 vs. age-matched AL rats.