Figure 4.

Analysis of brain-tropic and non-brain-tropic lung adenocarcinoma cell lines identified by the MetMap project showed differentially altered pathways and genes in common with lung cancer patient data. (a) Among the 48 lung adenocarcinoma cell lines analyzed by the MetMap project, 22 were from primary tumors, and among them 11 were found to have substantial metastatic potential. Five of these 11 were found to have a higher brain metastasis potential, while 6 were considered to have a low brain metastasis potential. (b) Analysis of cell line RNA-seq data from the CCLE database showed that the brain-tropic and non-brain-tropic cell lines have 1079 differentially expressed genes with an at least 2-fold expression difference and a p value lower than 0.05. The GO enrichment analysis (c) and the KEGG pathway enrichment analysis (d) showed multiple differences between the two groups of cell lines; the representative GO terms or KEGG pathways that were also identified in our patient cohort analysis were highlighted with red color. (e) Twenty-eight genes were found to be differentially expressed in the same direction in both the cell line analysis and the patient cohort analysis.