Figure 1.

Aerosol generation set-up. Engineered nanoparticles (ENP) aerosols were generated by controlled injection of the ENP aqueous suspensions by means of a syringe pump to a spray nozzle were the liquid was nebulised using pre-heated compressed air. This aerosol was further dried and mixed in a nebulising cylinder connected to the Vitrocell^® automated exposure station (AES). Just before entering the AES, a Teflon filter and a condensation particle counter (CPC) were connected for aerosol characterisation.