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. 2021 Dec 19;14(12):1330. doi: 10.3390/ph14121330

Figure 5.

Figure 5

SG2 does not show toxicological effects in zebrafish. (A) SG2 at 5 µM was administered to zebrafish embryos at 4 h post fertilization (hpf); the experimental workflow was created with BioRender.com( accessed on 5 May, 2021). All the experiments reported show non-significant values compared to controls, proving a safe impact of SG2 in zebrafish. (B,C) Mortality rate was calculated until 48 hpf (B) (controls N = 545, vehicle N = 281, SG2 N = 224) and no morphological anomaly was observed at 5 days post fertilization (dpf) (C). (D) Cardiotoxicity was evaluated measuring heart rate at 3 dpf. (E,F) Neurological development was evaluated at 30 hpf with burst activity analysis (E) and at 5 dpf with locomotor measurements considering velocity and distance covered (F) (controls N = 366, vehicle N = 212, SG2 N = 246). All experiments were performed at least in triplicate. Error bars represent the SEM. For statistical tests non-parametric one-tailed Mann-Whitney rank sum test was used.