Figure 6.

Surface hydrophobic patch of AlphaFold2 predicted structures of native chemokine receptors CCR10, CXCR5, CXCR7, and OR1D2 and their water-soluble QTY variants. The AlphaFold2 predicted structure receptors CCR10, CXCR5, CXCR7, and OR1D2 are presented. The large surface hydrophobic patch (yellow color) of the native receptors determined by AlphaFold2 predicted structures (cyan color): (A) CCR10, (B) CXCR5, (C) CXCR7, and (D) OR1D2. The hydrophobic patch is significantly reduced on the transmembrane domains for the AlphaFold2 predicted water-soluble QTY variants: (E) CCR10^QTY, (F) CCXC5^QTY, (G) CXCR7^QTY, and (H) OR1D2^QTY. These QTY variants become water-soluble without any detergent. The N- and C-termini are removed for clarity of directly comparisons.