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. 2021 Dec 6;11(12):1310. doi: 10.3390/jpm11121310

Figure 5.

Figure 5

Exosomes isolated from RR cells can alter the resistant phenotype of CSCs. (A) Chemosensitivity to increasing doses of doxorubicin was determined for (i) REM134, (ii) REM134 RR, (iii) MDA-MB-231 and (iv) MDA-MB-231 RR CSCs. CSCs were pretreated for 24 h with 50 μg/mL exosomes isolated from either non-RR or RR corresponding cell lines prior to treatment with the indicated dose of doxorubicin. Cell viability was assayed 72 h after doxorubicin treatment. (B) Colony-forming ability after treatment with 0, 2.5 or 5 Gy was determined for (i) REM134, (ii) REM134 RR, (iii) MDA-MB-231 and (iv) MDA-MB-231 RR CSCs. All CSCs were pretreated with 50 μg/mL of exosomes isolated from either non-RR or RR corresponding cell lines for 24 h prior to irradiation. Three repeats were performed, and data were analysed by a two-sample t test. Error bars indicate ±SD. * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.00001.