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. 2021 Dec 14;13(12):898. doi: 10.3390/toxins13120898

Figure 1.

Figure 1

Effects of TSE on NO production, cell viability, and the expression levels of iNOS in BV2 microglial cells. The cells were pre-treated with TSE (10, 30, or 100 μg/mL) or quercetin (10 μM) for 1 h before LPS treatment for 23 h. Cell viability was assessed by MTT assay (A). The cell culture supernatant containing NO was evaluated using Griess reagent (n = 3 per group) (B). The mRNA levels of iNOS were quantified using qRT-PCR (n = 3 per group) (C). GAPDH was used as an internal control. The protein levels of iNOS were measured using western blotting in BV2 cell lysates. The representative band of iNOS is shown (D). The quantification of iNOS level was normalized to β-actin (n = 4 per group). Data were analyzed by one-way ANOVA, followed by Dunnett’s post hoc test. *** p < 0.001 and **** p < 0.0001.