Figure 5.

Effects of TSE on the expression of HO-1 in BV2 microglial cells. The cells were pre-treated with TSE at 100 μg/mL for 1 h before LPS treatment, and were treated with LPS for 23 h. The mRNA levels of HO-1 (A) were quantified using qRT-PCR (n = 3 per group). GAPDH was used as an internal control. The protein levels of HO-1 were measured using western blotting in BV2 cell lysates. The representative band of HO-1 is shown (B). Quantification of HO-1 level was normalized to β-actin (n = 3 per group) (C). Data were analyzed by one-way ANOVA, followed by Dunnett’s post hoc test. ** p < 0.01, *** p < 0.001.