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. 2021 Dec 20;22(24):13646. doi: 10.3390/ijms222413646

Figure 5.

Figure 5

Excessive NET formation by postpartum circulating neutrophils is complemented by activation of the coagulation cascade and alterations in the platelet activity status. (a) Immunohistochemical staining for TF (green), and DNA counterstain with DAPI (blue) after a 3 h in vitro culture. Magnification: 20×; Scale bars: 50 μm. (b) Platelet differential counts in healthy non-pregnant blood donors, donors during pregnancy and 48 h postpartum. (c) Comparative analysis between healthy donor controls, donors during gestation and postpartum, concerning the platelet morphology and activation indices mean platelet volume (MPV) and platelet distribution width (PDW). (d) TAT complexes concentration in citrate plasma from healthy donor controls, donors during pregnancy and postpartum, determined by ELISA. (e) Spearman correlation between TAT complexes concentration and NET-associated MPO/DNA complexes. (f) D-dimer levels in citrate plasma from healthy donor controls, donors during gestation and postpartum, determined by ELISA. P: pregnancy; PD: post-delivery. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01 (one way ANOVA followed by Bonferroni’s multiple comparison post-test). All experiments were performed at least 6 times with consistent results.