Table 2.
General characteristics of the experimental groups.
| p Values for Two-Way ANOVA | |||||||
|---|---|---|---|---|---|---|---|
| Characteristic | Control +Sed |
ISPG +Sed |
Control +RT |
ISPG +RT |
Main Effect of RT | Main Effect of ISPG | Interaction (RT × ISPG) |
| Initial BW (g) | 30.14 ± 0.38 | 30.24 ± 0.27 | 30.11 ± 0.42 | 30.29 ± 0.42 | 0.9740 | 0.7180 | 0.9220 |
| Final BW (g) | 37.28 ± 0.41 | 37.26 ± 0.81 | 37.65 ± 1.15 | 37.34 ± 0.25 | 0.7650 | 0.8290 | 0.8420 |
| Food intake (g) | 7.49 ± 0.31 | 6.95 ± 0.23 | 7.81 ± 0.30 | 6.99 ± 0.28 | 0.5220 | 0.0160 * | 0.6150 |
| Water intake (mL) | 7.72 ± 0.09 ab | 6.95 ± 0.05 a | 7.76 ± 0.08 ab | 7.91 ± 0.10 b | 0.0050* | 0.6180 | 0.0250 * |
| Tissue weight (g) | |||||||
| Liver | 2.06 ± 0.05 | 2.07 ± 0.09 | 2.17 ± 0.06 | 2.07 ± 0.07 | 0.4250 | 0.5430 | 0.4660 |
| Gastrocnemius muscle | 0.39 ± 0.02 | 0.40 ± 0.01 | 0.40 ± 0.02 | 0.41 ± 0.01 | 0.4730 | 0.5260 | 0.8990 |
| Quadriceps | 0.48 ± 0.02 a | 0.53 ± 0.01 a | 0.54 ± 0.02 a | 0.55 ± 0.01 b | 0.0220 * | 0.0360 * | 0.2890 |
| Heart | 0.19 ± 0.01 | 0.19 ± 0.01 | 0.19 ± 0.01 | 0.19 ± 0.00 | 0.8440 | 0.5560 | 0.5560 |
| Lung | 0.23 ± 0.01 | 0.23 ± 0.01 | 0.24 ± 0.01 | 0.24 ± 0.01 | 0.5030 | 0.9110 | 0.5770 |
| Kidney | 0.75 ± 0.04 | 0.79 ± 0.03 | 0.78 ± 0.03 | 0.75 ± 0.03 | 0.8930 | 0.8620 | 0.2940 |
| EFP | 0.51 ± 0.04 b | 0.34 ± 0.05 a | 0.31 ± 0.03 a | 0.33 ± 0.03 a | 0.0130 * | 0.0620 | 0.0190 * |
| BAT | 0.11 ± 0.01 | 0.11 ± 0.01 | 0.11 ± 0.01 | 0.12 ± 0.01 | 0.4820 | 0.4820 | 0.4820 |
| Relative tissue weight (%) | |||||||
| Liver | 5.53 ± 0.14 | 5.54 ± 0.18 | 5.78 ± 0.17 | 5.55 ± 0.17 | 0.4160 | 0.5150 | 0.4690 |
| Gastrocnemius muscle | 1.04 ± 0.04 | 1.06 ± 0.03 | 1.05 ± 0.03 | 1.09 ± 0.04 | 0.4880 | 0.4070 | 0.8350 |
| Quadriceps | 1.28 ± 0.06 a | 1.43 ± 0.02 b | 1.42 ± 0.03 b | 1.48 ± 0.04 b | 0.0150 * | 0.0130 * | 0.2780 |
| Heart | 0.50 ± 0.02 | 0.51 ± 0.02 | 0.52 ± 0.03 | 0.50 ± 0.01 | 0.9760 | 0.6140 | 0.5730 |
| Lung | 0.61 ± 0.04 | 0.63 ± 0.02 | 0.65 ± 0.03 | 0.63 ± 0.03 | 0.5770 | 0.9050 | 0.6610 |
| Kidney | 2.00 ± 0.07 | 2.11 ± 0.06 | 2.07 ± 0.08 | 2.01 ± 0.08 | 0.8230 | 0.7570 | 0.2350 |
| EFP | 1.37 ± 0.09 b | 0.89 ± 0.12 a | 0.83 ± 0.08 a | 0.89 ± 0.07 a | 0.0070 * | 0.0370 * | 0.0070 * |
| BAT | 0.29 ± 0.01 | 0.29 ± 0.02 | 0.29 ± 0.02 | 0.31 ± 0.01 | 0.4820 | 0.4820 | 0.5260 |
All data are expressed as the mean ± SEM of 8 mice/group. Different letters (a, b) in the same column of data are significantly different, p < 0.05. If any letter is the same, it means that the difference between groups is not significant. The main effect is analyzed by two-variance analysis, * p < 0.05. EFP: epididymal fat pad; BAT: brown adipose tissue.