Table 1.

Primer sequence, product size, annealing temperature, for quantitative RT-PCR.

Target Gene (Abbreviation)	Forward (Top) and Reverse (Bottom) Primer Sequences	Size of Amplified Products (bp)	Annealing Temperature (°C)	Sequence ID
β-actin	5’-ATTGCCGACAGGATGCAGA-3’ 5’-GAGTACTTGCGCTCAGGAGGA-3’	89	60	NM_001101.3
ALK1	5’-ATCCTAGGCTTCATCGCCTC-3’ 5’-TAGCCTCAGAGCCAGATGGG-3’	141	60	NM_000020.3
ALK2	5’-AGGCTGCTTCCAGGTTTATGAG-3’ 5’-TGGCAGCACTCCACAGCTT-3’	81	60	NM_001105.5
ALK3	5’-AGTGTCTCCAGTCAAGCTCTGGGTA-3’ 5’-CCATCTCTGCTGCGCTCATTTA-3’	97	60	NM_004329.3
ALK4	5’-TGCTGCGCCATGAAAACATC-3’ 5’-GGGACCCGTGCTCATGATAG-3’	105	60	NM_020327.3
ALK5	5’-CCTTCTGACCCATCAGTTGAAGA-3’ 5’-CCTAGCTGCTCCATTGGCAT-3’	150	60	NM_004612.4
ALK6	5’-GTTACGCCCCTCATTCCCAA-3’ 5’-GTTTTCTTAACCCGCAGGGC-3’	126	60	NM_001256793.2
Inhibinβa	5’-TGTGCCCACCAAGCTGAGAC-3’ 5’-CTGGGCTGGGCAACTCTATGA-3’	124	60	NM_002192.2
OCN	5’-GTGCAGAGTCCAGCAAAGGT-3’ 5’-TCAGCCAACTCGTCACAGTC-3’	110	60	NM_199173.4
OPN	5’-ACACATATGATGGCCGAGGTGA-3’ 5’-TGTGAGGTGATGTCCTCGTCTGT-3’	115	60	NM_000582.3
RUNX2	5’-AACCCTTAATTTGCACTGGGTCA-3’ 5’-CAAATTCCAGCAATGTTTGTGCTAC-3’	145	60	NM_001024630.3