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. 2021 Dec 13;13(12):4455. doi: 10.3390/nu13124455

Figure 2.

Figure 2

Sesamin promoted BMSCs osteogenesis via up-regulating Wnt/β-catenin signaling pathway. (A). Cytotoxicity assay of mouse BMSCs after treated with sesamin at different concentration for 3 days (n = 6; * p < 0.05, *** p < 0.001). (B). The ALP staining at Day 3 and the ARS staining of calcified nodules at Day 7 after osteo-induction were performed. (C). The mRNA expression level of osteogenic marker genes were detected by quantitative real-time PCR after treatment with sesamin after osteo-induction for 7 days (n = 6; * p < 0.05, ** p < 0.01, *** p < 0.001). (D). Sesamin up-regulated the expression of β-catenin in mouse BMSCs at both mRNA and protein expression levels (n = 6; * p < 0.05, ** p < 0.01). (E). The mRNA expression level of Wnt/β-catenin downstream genes in mouse BMSCs were significantly increased after sesamin treatment at different concentrations (n = 6; ** p < 0.01, *** p < 0.001).