Figure 3.

Sesamin inhibited osteoclastogenic differentiation of RAW264.7 cell by deactivating NF-κB signaling pathway. (A). Cytotoxicity assay of RAW264.7 cells after treated with sesamin at different concentration for 3 days (n = 6; * p < 0.05, ** p < 0.01). (B). TRAP staining assay at Day 5 after RANCL-induced osteoclastogenesis was performed. (C). Quantitative data of osteoclast fold change (n = 6; * p < 0.05, ** p < 0.01). (D). The mRNA expression level of osteoclastogenic differentiation related markers measured by qRT-PCR (n = 6; * p < 0.05, ** p < 0.01, *** p < 0.001). (E). Analysis of NF-κB/p65 nucleus translocation and IκBα phosphorylation levels by Western blot analysis in RAW264.7 cells after cells were treated with sesamin for 24 h and treated with RANKL for 30 min.