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. 2021 Dec 13;13(12):4455. doi: 10.3390/nu13124455

Figure 4.

Figure 4

Knockdown of DANCR attenuated the Osteogenic effect of sesamin. (A). DANCR expression level decreased in BMSCs upon sesamin treatment (n = 6; * p < 0.05, ** p < 0.01). (B,C). DANCR knockdown upregulated the mRNA (B) and protein (C) expression level of β-catenin (n = 6; ** p < 0.01). (D,E). The BMSCs were DANCR knockdown and treated with sesamin during osteo-induction for 7 days. ALP activity, calcified nodules formation (D) and mRNA expression level of osteogenic marker genes (E) were examined by ALP activity assay, ARS staining and real-time PCR, respectively (n = 6; * p < 0.05, ** p < 0.01, *** p < 0.001, versus si-NC+DMSO group; ^ p < 0.05, versus si-DANCR+DMSO group). (F,G). Sesamin up-regulated the expression of β-catenin in mouse BMSCs at both mRNA (F) and protein (G) expression levels, this upregulation was attenuated upon si-DANCR treatment (n = 6; ** p < 0.01, *** p < 0.001).