Table 6.
Primers used for quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) analysis for study II using broiler's samples.
| Gene1 | Gene bank identification | Primer sequence, sense/antisense |
|---|---|---|
| Housekeeping genes | ||
| GAPDH | NM_204305.1 | GCTAAGGCTGTGGGGAAAGT/ TCAGCAGCAGCCTTCACTAC |
| Beta-actin | NM_205518.1 | CAACACAGTGCTGTCTGGTGGTA/ ATCGTACTCCTGCTTGCTGATCC |
| Tight junction protein genes | ||
| Cla-1 | NM_001013611.2 | TGGAGGATGACCAGGTGAAGA/ CGAGCCACTCTGTTGCCATA |
| Cla-2 | NM_001277622.1 | CCTGCTCACCCTCATTGGAG/ GCTGAACTCACTCTTGGGCT |
| ZO-1 | XM_015278981.2 | CAACTGGTGTGGGTTTCTGAA/ TCACTACCAGGAGCTGAGAGGTAA |
| ZO-2 | XM_025144669.1 | ATCCAAGAAGGCACCTCAGC/ CATCCTCCCGAACAATGC |
| Ocln | XM_026041453.1 | ACGGCAGCACCTACCTCAA/ GGCGAAGAAGCAGATGAG |
| JAM2 | XM_025149444.1 | AGCCTCAAATGGGATTGGATT/ CATCAACTTGCATTCGCTTCA |
1
Abbreviations: Cla-1, Claudin-1; Cla-2, Claudin-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Ocln, Occludin; JAM2, junctional adhesion molecule 2; ZO-1, Zonula Occludens-1; ZO-2, Zonula Occludens-2.