Table 7.

Relative mRNA expression of genes related to tight junction proteins in jejunum of broiler chickens fed low glucosinolate carinata meal (LGCM) and high glucosinolate carinata meal (HGCM) from study II.

Items/genes1	Cla-1	Cla-2	JAM-2	Ocln	ZO-1	ZO-2
Control	1	1	1	1	1	1
4% LGCM	0.93	0.88	0.98	0.97	0.89	1.05
8% LGCM	0.83	0.63	0.93	1.02	0.91	0.96
12% LGCM	1.14	0.73	0.96	1.21	1.13	1.04
4% HGCM	1	0.83	0.79	1.16	0.86	0.94
8% HGCM	0.88	0.78	0.82	1.11	0.91	0.97
12% HGCM	1.41	0.71	0.98	1.12	1.05	1.16
SEM	0.085	0.057	0.049	0.034	0.033	0.029
P-values
LGCM linear	0.773	0.158	0.794	0.103	0.313	0.889
LGCM quadratic	0.416	0.479	0.824	0.230	0.071	0.859
HGCM linear	0.315	0.221	0.974	0.472	0.611	0.170
HGCM quadratic	0.279	0.763	0.211	0.418	0.120	0.135
Control vs LGCM	0.898	0.198	0.793	0.539	0.819	0.817
Control vs HGCM	0.741	0.260	0.439	0.257	0.577	0.794
LGCM vs HGCM	0.487	0.812	0.434	0.416	0.613	0.962

¹Genes: Cla-1: Claudin-1, Cla-2: Claudin-2, JAM2: junctional adhesion molecule-2, Ocln: Occludin, ZO-1: Zonula Occludens-1, ZO-2: Zonula Occludens-2.