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The preparation process of melatonin-loaded silk fibroin microneedle (MT/SF@MNs): the mixture was poured into a polydimethylsiloxane microneedle mold, evacuated 3 times to remove the bubbles, and then dried in a constant temperature and humidity room for 8 h (25 °C, 55%RH) and demolded to obtain MNs.
