Figure 4.

ATP and Ca²⁺ quantification and ROS content in CT and RU spermatozoa. (A) Ca²⁺ assay in spermatozoa of CT (n = 5) and RU (n = 5) roosters at Days 0, 13, 25 and 50 (14 days after the end of RU exposure (n = 3 CT and n= 3 RU roosters) (mg/L). Stars (*) correspond to the unpaired t-test significance (p < 0.05). (B) Ratio between ATP concentration and protein concentration in spermatozoa of CT and RU roosters at Days 0, 13, 25 (n = 5 CT and n = 5 RU roosters at each time) and 50 (n = 3 CT and n = 3 RU roosters 14 days after the end of RU exposure). (C) Reactive Oxygen Species (ROS, H₂0₂) levels in spermatozoa (n = 5 CT and 5 RU animals at Day 36 and n = 3 CT and n = 3 RU animals at Day 50). Stars (*) correspond to the unpaired t-test significance (p < 0.05).