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. 2021 Dec 8;22(24):13233. doi: 10.3390/ijms222413233

Figure 3.

Figure 3

Effects of ABT-263 on the induction of apoptosis of irradiated cancer cells: (A,B) X-irradiated A549 and Ca9-22 cells were cultured for 24 h prior to the addition of ABT-263 to the culture (5 μM for A549 cells and 10 μM for Ca9-22 cells). After 24 h of culture, the cells including floating cells were harvested for apoptosis analysis. (A) Representative cytograms are shown. The inset numbers indicate the percentage of annexin V+/propidium iodine (PI)− cells or annexin V+/PI+ cells. (B) The net increase in annexin V+ apoptotic cells (sum of annexin V+/PI− cells and annexin V+/PI+ cells) by 6 Gy is shown. Data are presented as the mean ± SD of independent experiments. ** p < 0.01.