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. 2021 Dec 8;22(24):13233. doi: 10.3390/ijms222413233

Figure 5.

Figure 5

ABT-263 induces caspase-mediated apoptosis of irradiated A549 cells. (A) A549 cells irradiated at 6 Gy were cultured for 24 h prior to the addition of ABT-263 (5 μM) to the culture. After 24 h of culture, the cells were harvested for western blot analysis. β-actin was used as a loading control. Representative immunoblots are shown. (B) A549 cells irradiated at 6 Gy were cultured for 24 h prior to the addition of ABT-263 to the culture. Z-VAD was added to the culture 1 h before ABT-263 administration. After 48 h of culture, the cells including floating cells were harvested for apoptosis analysis. Representative cytograms are shown. The inset numbers of the cytograms indicate the percentage of annexin V+/PI− cells or annexin V+/PI+ cells.