Figure 3.

Workflow of single-cell RNA sequencing. The first step is the tissue dissociation into a cell suspension. The single-cell is lysed after isolation, and the RNA is collected and transcribed to cDNA via reverse transcription. After that, the cDNA is amplified to create a high-throughput RNA-seq library. The analysis of single-cell expression profiles permits the identification of different cell populations and subpopulations. The scRNA-seq allows for the characterizing of immune cells within the tumor microenvironment, as well as cells present in low numbers and in the quiescent state (https://smart.servier.com accessed on 23 August 2021).