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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Before corneal staining, (A) the positions of the six zones (Ra–Rf, La–Lf) made on the isolated canine cornea. The black arrow indicates the corneoscleral limbus. Photoshop software was used to stitch (B) the cornea staining maps in each zone and draw (C) the nerve direction.