Table 2.
An overview of statistics for different E. coli and K. pneumoniae assemblies produced by the top-performing assemblers. IllumASM was produced using Unicycler, MinIONASM using Flye and HybASM created using Unicycler. The top values are highlighted in bold. The mixed culture was obtained from the co-culturing of E. coli 4 and K. pneumoniae 5 isolates. Numbers show the average ± SD.
| Number of Dead Ends | Number of Contigs | Total Length (bp) | N50 (bp) | ||
|---|---|---|---|---|---|
| E. coli | IllumASM | 4 ± 4 | 138 ± 90 | 5,232,982 ± 335,084 | 225,244 ± 82,435 |
| MinIONASM | 77 ± 94 | 49 ± 47 | 3,870,499 ± 2,664,510 | 343,234 ± 504,598 | |
| HybASM | 4 ± 2 | 50 ± 28 | 5,317,286 ± 426,129 | 1,005,273 ± 476,961 | |
| K. pneumoniae | IllumASM | 10 ± 7 | 78 ± 13 | 5,577,253 ± 181,931 | 247,095 ± 138,114 |
| MinIONASM | 44 ± 48 | 35 ± 32 | 4,694,978 ± 2,235,357 | 1,996,101 ± 2,279,327 | |
| HybASM | 1 ± 3 | 20 ± 17 | 5,648,111 ± 211,443 | 3,880,248 ± 2,149,256 | |
| Mixed culture sample | IllumASM | 2 | 371 | 11,193,506 | 147,235 |
| MinIONASM | 65 | 120 | 11,827,293 | 344,695 | |
| HybASM | 0 | 117 | 11,495,693 | 1,245,846 |