Fig. 1.

Isolation and bioinformatics analysis of SARS-CoV-2 Beta variant sequences: (A) cytopathic effect observed on the Vero CCL-81 cell line for the 3rd passage at 3rd PID along with the control (left). (B) Neighbor joining tree of the isolate sequences with reference sequences downloaded from GISAID with Tamura’s 3 Parameter model and a bootstrap replication of 1000 cycles. The sequences retrieved in study are colored red. (D) SARS-CoV-2 Beta variant sequences retrieved in this study are aligned with reference isolate of Wuhan-HU-1 (Accession No.: NC_045512.2). The nucleotide changes are generated using the highlighter plot available at https://www.hiv.lanl.gov/content/sequence/HIGHLIGHT/highlighter_top.htm.

The mismatches are marked in different colors adenine (A) green; cytosine (C) blue; guanine (G) orange, thymine (T) red and gap black color.