Fig. 2.

Nucleotide changes of SARS-CoV-2 for two isolate in different genes: (A) the viral copy number for the isolates at passage 2–5 levels using real-time RT-PCR. (B) The nucleotide changes for the isolate MCL-19-H-893 is marked in the upper panel for passage 2–5 and for isolate MCL-19-H-1493 in the lower panel. The x axis represents the different passages and y axis depicts the frequency of their observation. A cut-off of 50% defines the consensus sequences nucleotide changes while below it are quasi-species for the isolates. Non-synonymous changes are marked in red color; synonymous changes are marked in green color; deletions of nucleotide are marked in black color.