Figure 3.
VEGF increases vessel maturity, stability, and vessel diameter. (a) Representative images of CD31 staining of vasculature surrounding −VEGF and +VEGF devices. Scale bar = 200 μm. (b) Number of blood vessels per mm2. (c) Length density. (d) Radial diffusion distances. (e) Representative fluorescent images of αSMA (green) and CD31 (red) staining of fibrous capsules for −VEGF and +VEGF samples. Scale bar = 50 μm. (f) Percentage of αSMA+ vessels for analysis of vessel stability and maturity. (g) Blood vessel diameters (h) Percentage frequency distribution of blood vessel diameters surrounding −VEGF and +VEGF devices. n = 4 per group, data are represented as means ± SD, ** p < 0.01, *** p < 0.001.