Table 2.
Studies of mice inoculations with vaccine candidates.
| Study | Group | # of Mice/Group |
Age of Mice (Weeks) | Sex of Mice | Prime | Prime Dose (pfu or µg) | Prime Adjuvant | Boost | Boost Dose(pfu or µg) | Boost Adjuvant | Bleed & Boost Schedule (Weeks Post Prime) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 4 | 15–20 | F M | PBS | N/A | N/A | PBS | N/A | N/A | 2—bleed 2—boost 4—bleed 4—boost 6—bleed 8—bleed |
| 2 | 4 | 15–20 | F M | phiTM45 | 1E10 | N/A | phiTM45 | 1E10 | N/A | ||
| 3 | 4 | 15–20 | F M | phiTM45 | 1E11 | N/A | phiTM45 | 1E11 | N/A | ||
| 4 | 4 | 15–20 | F M | BaDAS-1 | 1E10 | N/A | BaDAS-1 | 1E10 | N/A | ||
| 5 | 4 | 15–20 | F M | BaDAS-1 | 1E10 | 5 µg LPS | BaDAS-1 | 1E10 | 5 µg LPS | ||
| 6 | 4 | 15–20 | F M | BaDAS-1 | 1E11 | N/A | BaDAS-1 | 1E10 | N/A | ||
| 2 | 1 | 5 | 8 | F | PBS | N/A | N/A | PBS | N/A | N/A | 3—bleed 3—boost 6—bleed 9—bleed 12—bleed |
| 2 | 5 | 8 | F | Spike S1 | 20 | 5 µg LPS | Spike S1 | 20 | 5 µg LPS | ||
| 3 | 5 | 8 | F | phiTM45 | 1E11 | N/A | phiTM45 | 1E11 | N/A | ||
| 4 | 5 | 8 | F | phiTM45 | 1E11 | 5 µg LPS | phiTM45 | 1E11 | 5 µg LPS | ||
| 5 | 5 | 8 | F | BaDAS-1 | 1E11 | N/A | BaDAS-1 | 1E11 | N/A | ||
| 6 | 5 | 8 | F | BaDAS-1 | 1E11 | 5 µg LPS | BaDAS-1 | 1E11 | 5 µg LPS | ||
| 7 | 5 | 8 | F | BaDAS-3 | 1E11 | N/A | BaDAS-3 | 1E11 | N/A | ||
| 8 | 5 | 8 | F | BaDAS-3 | 1E11 | 5 µg LPS | BaDAS-3 | 1E11 | 5 µg LPS | ||
| 9 | 5 | 8 | F | BADAS-4 | 1E11 | N/A | BADAS-4 | 1E11 | N/A | ||
| 10 | 5 | 8 | F | BaDAS-4 | 1E11 | 5 µg LPS | BaDAS-4 | 1E11 | 5 µg LPS | ||
| 3 | 1 | 3 | 12 | F M | BADAS-5 | 5E10 | N/A | N/A | N/A | N/A | 2—bleed 4—bleed |
| 2 | 3 | 12 | F M | BaDAS-6 | 5E10 | N/A | N/A | N/A | N/A | ||
| 3 | 3 | 12 | F M | BaDAS-7 | 5E10 | N/A | N/A | N/A | N/A | ||
| 4 | 1 | 3 | 12 | F | BaDAS-1 | 5E10 | N/A | BaDAS-1 | 5E10 | N/A | 3—bleed 3—boost 6—bleed 9—bleed |
| 2 | 3 | 12 | F | BaDAS-1 | 5E10 | N/A | Spike S1 | 10 | N/A | ||
| 3 | 3 | 12 | F | BaDAS-5 | 5E10 | N/A | BaDAS-5 | 5E10 | N/A | ||
| 4 | 3 | 12 | F | BaDAS-5 | 5E10 | N/A | Spike S1 | 10 | N/A | ||
| 5 | 1 | 3 | 8 | F M | RBD | 2 | 2.5 µg LPS | RBD | 2 | 2.5 µg LPS | 3—bleed 3—boost 6—bleed 9—bleed |
| 6 | 1 | 3 | 12 | F | DEaDAS-1 | 5E9 | N/A | DEaDAS-1 | 1E11 | N/A | 3—bleed 6—bleed 11—boost 14—bleed |
| 7 | 1 | 4 | 8 | F | PBS | N/A | N/A | Spike S1 | 10 | N/A | 3—bleed 6—boost 9—bleed |
| 2 | 4 | 8 | F | BaDAS-1 | 1E11 | N/A | BaDAS-1 + Spike S1 |
1E11 + 10 |
N/A | ||
| 3 | 4 | 8 | F | DEaDAS-1 | 1E11 | N/A | Spike S1 | 10 | N/A |