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Live-cell imaging of ER–EL contacts in U2OS cells overexpressing SNX13-GFP 10 min after incubation in hypotonic media. Yellow arrowheads indicate ER–EL contacts. Contact length is displayed in white. Images were acquired every 15 s; total video length is 10 min. Scale bars, 10 µm; enlarged insets, 1 µm. Video plays at 15 seconds/frame.
