TABLE 1.
Telomere FISH analyses of GM847 and GM847/hTERT cells
| Cell type | Metaphases with at least one undetectable telomere (%)a | No. of undetectable telomeres (%)b |
|---|---|---|
| GM847 untransfected | 50/50 (100) | 41/704 (6) |
| GM847/hTERT-3 (PD 42) | 12/50 (24) | |
| GM847/hTERT-3 (PD 106) | 9/50 (18) | 8/690 (1) |
| GM847/hTERT-3 (PD 138) | 13/50 (26) | |
| GM847/hTERT-6 (PD 46) | 13/55 (23) | |
| GM847/hTERT-6 (PD 82) | 15/50 (30) | 1/894 (0.1) |
| GM847/hTERT-6 (PD 150) | 13/52 (25) | |
| GM847/hTERT-3 subclone 9 | 1/50 (2) | 2/1,232 (0.2) |
| GM847/hTERT-6 subclone 11 | 0/50 (0) | 2/1,210 (0.2) |
a
Metaphases were scored as containing at least one chromosome end without a detectable telomere FISH signal.
b
Number of undetectable telomeres relative to the total number of chromosome ends analyzed by scoring every chromosome end in five additional (i.e., different) metaphases.