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. 2021 Dec 23;9(12):e002843. doi: 10.1136/jitc-2021-002843

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Treatment of mice with VV-scFv-TIGIT established long-term tumor-specific immunological memory. (A) Rechallenge scheme. (B) Kaplan-Meier survival curves of the treatment-naïve mice or the mice cured of H22 by VV-scFv-TIGIT. (C) Tumor volumes of previously cured mice rechallenged with H22 or MC38 cells subcutaneously. (D) Rechallenge scheme and representative diagram of flow cytometric analysis of memory T cells. (E) Flow cytometric analysis of the proportion of CD8⁺ T cells, naïve (CD62L⁺CD44⁻), effector memory (CD62L^-CD44⁺) and central memory (CD62L⁺CD44⁺) CD8⁺ T cells. (F) Co-culture of H22 cells with splenocytes from the H22 rechallenged mice. The proportion of lymphocytes and tumor cells in the co-culture system was detected by flow cytometry. The levels of cytokines in the co-culture system were detected by ELISA. ns, no significant differences; *p<0.05; ***p<0.001; ****p<0.0001. scFv, single-chain variable fragment; TIGIT, T-cell immunoglobulin and ITIM domain; VV, vaccinia virus.