Fig. 5.

Inflamed pericytes facilitate transmigration of macrophages in vitro. A Using the Boyden chamber assay, we treated bone marrow derived macrophages (BMDMs) with culture media (CM) from either 10 ng/mL IL-1β + IFN-γ or 10 µg/mL CSPG-treated pericytes and compared with the potential of untreated pericyte media. B Brightfield images of fixed membranes showing BMDMs treated with the inflammatory cocktail as well as the CSPGs transmigrated significantly higher than the BMDMs from untreated pericytes, which is quantified in histograms to the right. Data are represented as mean ± SEM of quadriplicate analyses. **p < 0.01