Table 3.

NS2A and NS4B combination assay.

Protein	EC50 (ng/well)	FIC50 A/B 1	FIC50 1	Combination Effect
NS2A (A)	40.15	0.35	0.5	Synergism
NS4B (B)	93	0.15

¹ FIC₅₀ values were determined according to the fixed-ratio method of Fivelman et al. [27]. FIC₅₀ < 1—synergistic action; FIC₅₀ = 1—additive action; FIC₅₀ > 1—antagonistic action [26].