DEPC-treated water	6 µL
5x TranscriptAid reaction buffer	4 µL
ATP, Tris buffered, 30 mM	1 µL (1.5 mM final concentration)
CTP, Tris buffered, 100 mM	1.5 µL (7.5 mM final concentration)
GTP, Tris buffered, 100 mM	1.5 µL (7.5 mM final concentration)
UTP, Tris buffered, 100 mM	1.5 µL (7.5 mM final concentration)
Cap analog G(5′)ppp(5′)A, 100 mM	1.2 µL (6 mM final concentration)
Co25 template DNA, double stranded, preheated at 95 °C for 10 min, then slowly cooled down to 35 °C	1 µg (0.5 µL)
TranscriptAid enzyme mix	2 µL