Table 3.

Histological alterations in the liver, lungs, and kidneys tissues of immunosuppressed mice inoculated with the S. schenckii sensu stricto and S. brasiliensis, observed with hematoxylin-eosin and Grocott’s methenamine silver (GMS) preparations at different time points.

Days after Inoculation	Sporothrix Species		Organs
			Kidney	Liver	Lungs
21	S. schenckii sensu stricto	ABSENT		ABSENT	ABSENT
	S. brasiliensis	ABSENT		ABSENT	ABSENT
35	S. schenckii sensu stricto	Interstitial nephritis, granulomatous, multifocal and severe in the capsule, cortex, medulla and pelvis. Multifocal areas of fibrosis. Poorly organized granuloma. GMS: abundant rounded yeasts with a narrow-based budding or cigar-shaped in in the capsule, cortical, medullary region and renal pelvis.		Hepatitis, pyogranulomatous, necrotizing, multifocal and moderate to severe. Well-organized granulomas. GMS: abundant rounded yeasts with a narrow-based budding or cigar-shaped, amid the inflammatory infiltrate	Pneumonia, pyogranulomatous, multifocal and moderate to severe. Abundant yeast-like structures amid the inflammatory infiltrate. GMS: abundant rounded yeasts with a narrow-based budding or cigar-shaped, amid the inflammatory infiltrate
	S. brasiliensis	ABSENT		ABSENT	ABSENT
49	S. schenckii sensu stricto	All animals had died by this point in the study, and histopathological analysis was not possible.
	S. brasiliensis	ABSENT		ABSENT	Tissue alterations were not detected by HE staining. GMS: rounded yeasts with a narrow-based budding or cigar-shaped, amid the inflammatory infiltrate
	CONTROL (of all species at different times)	ABSENT		ABSENT	ABSENT

ABSENT: Tissue alterations and yeasts of Sporothrix spp. were not detected by hematoxylin-eosin and Grocott’s methenamine silver (GMS) preparations.