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6MMPr induces accumulation of PRPP. Endogenous levels of PRPP were quantified by LC/MS/MS and expressed as total picomoles (pmol) for samples for both nucleobase (T-1105) (A) and nucleoside (T-1106) (B) experiments. PRPP levels were adjusted vs. the total amount of protein from each sample. The mean ± SD of two independent experiments were used to build the graphs. *, p < 0.05; **, p < 0.005; ***, p < 0.001.
