Figure 8.

6MMPr enhances antiviral effect of nucleobase but not nucleoside in ZIKV-infected cells. Bar graphs represent the viral titer reduction induced by favipiravir (FAV)/6MMPr (A), T-1105/6MMPr (C), and T-1106/6MMPr (E) co-treatments in Huh7 cells inoculated with wild type ZIKV. Mean ± SEM from two independent experiments conducted in duplicate are plotted. “a” indicates significant antiviral activity induced by the nucleobase or the nucleoside alone. * p < 0.05; ** p < 0.005. Black dotted line indicates limit of detection. (B,D,F) show the 3D plots indicating synergistic or additive antiviral effect. Total volume of synergy = TVS. All TVS calculated at 99.9% confidence interval.