FIG. 1.

PTEN induces a G₁ cell cycle arrest followed by apoptosis. MDA-MB-468 cells were infected with either Ad-PTEN or Ad-β-gal (control) to examine the effects of PTEN on cell cycle and survival. (A) Cells infected with either Ad-PTEN or Ad-β-gal were collected at the indicated time points postinfection and subjected to flow-cytometric analysis as described in Materials and Methods. The graph shows the ratio of cells in G₁ to cells in S and G₂ phase of the cell cycle versus time points postinfection. (B) Cells infected with either Ad-PTEN or Ad-β-gal were collected at 40 h postinfection and subjected to flow-cytometric analysis to measure DNA content.