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IRS-2 protein is upregulated by PTEN. MDA-MB-468, BT-549, and HBL100 cell lines were infected with Ad-PTEN, and cell lysates were collected at various time points postinfection (hours p.i.). (A) Immunoblot analysis of MDA-MB-468 cell lysates with antibodies to PTEN, IRS-2, and tubulin. (B) Immunoblot analysis of BT-549 and HBL100 cell lines with antibodies to PTEN, phospho-Akt, IRS-2, and tubulin.
