FIG. 5.

LY294002 upregulates IRS-2 RNA and protein levels in two breast cancer cell lines. MDA-MB-468 and BT-549 cells were treated with DMSO (control) or LY294002 or were left untreated for 3 h. (A) RNA was collected from treated cells as described previously and subjected to Northern blot analysis. Blots were hybridized with ³²P-labeled IRS-2 probe, stripped, and hybridized with GAPDH probe as a control. U, untreated; D, DMSO. (B) Total cell lysates were collected and subjected to immunoblot analysis using antibodies to PTEN, phospho-Akt, and tubulin.