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. 2021 Dec 17;22(24):13573. doi: 10.3390/ijms222413573

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Ovariectomy and E2 treatment effects on (A) inflammatory adipokine expression profile and (B) markers of apoptosis in WAT of female ZDF rats. Il, interleukin; Tnf, tumor necrosis factor alpha; Serpine1, plasminogen activator inhibitor-1; Ccl3, macrophage inflammatory protein-1 alpha; Cd68, cluster of differentiation 68; Bad, Bcl-2-associated death promoter; Bcl2, B-cell lymphoma 2. mRNA levels were normalized to housekeeping Gapdh mRNA expression. Values are expressed as the mean ± SEM in arbitrary units (au) related to LEAN group. Differences between obese-diabetic (SHAM) and control (LEAN) animals were analyzed by Student’s t-test. Differences between obese-diabetic groups with different hormonal manipulation were analyzed by One-way ANOVA with Fisher’s LSD post-hoc test.