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. 2021 Dec 16;22(24):13521. doi: 10.3390/ijms222413521

Figure 3.

Figure 3

Cytotoxicity of THV_ADA and THV_ADA-SP. (a) The cytotoxic effect of R-LM113 (blue), THV_ADA (orange), and THV_ADA-SP (green) was assessed by Alamar Blue in SKOV3 infected at different MOI up to 13 days after infection. * and ** were, respectively, used to label p-value < 0.05 and <0.005. (b) Time-course evaluation (24, 72, 120 h post infection) by brightfield microscopy of THV_ADA-mediated cytopathic effect on SKOV3 cells infected at different MOI (0.01, 0.05, 0.1, 0.5 pfu/cell). Bar indicates 100 µm. (c) Transcriptomic analysis by Genevestigator of CD39, CD73, and ADO receptors in SKOV3 cells. (d) Expression of CD39 and CD73 in SKOV3 cells was determined by Western Blot analysis. B-Actin was used as control.