Table 2.
Primers for qPCR experiments in BRIN-BD11 cells.
| Gene | Forward Primer | Reverse Primer | Annealing Temperature |
|---|---|---|---|
| Actb | AAGACCTCTATGCCAACAC | TGATCTTCATGGTGCTAGG | 57–61° 1 |
| Rpl13a | GCACAAGACCAAAAGAGG | CGCTTTTTCTTGTCATAGGG | 57–61° 1 |
| Hk1 | AAACTCTGGGAAACAAAGG | AAACTTGGTCTCAAAGATGC | 57° |
| Hnf4a | TGTGTGAGTCTATGAAGGAG | ATGTAGTCATTGCCTAGGAG | 61° |
| Pdx1 | AAAGGTTACAAACTTGAGCG | AAACAGCTCCCTTTATTCTC | 59° |
| Srebf1 | AAACCTGAAGTGGTAGAAAC | TTATCCTCAAAGGCTGGG | 59° |
| Ins1 | AACGTGGTTTCTTCTACAC | TCTCCAGTTGGTAGAGGG | 61° |
| Abcg1 | GTTATGTTCTTTGATGAGCCC | CCTTGACTTAGGACATAAAGC | 57° |
| Abca1 | CATCTGAAAAACAGGTTTGG | GGGAGAGAATGCTGAATATC | 57° |
| Txnip | CGTCAATACTCCTGACTTAATG | AAATGTCATCACCTTCACAG | 61° |
1 Annealing temperature for reference genes varied to match that of the gene of interest in each assay.