Skip to main content
. 2021 Nov 23;13(12):4195. doi: 10.3390/nu13124195

Figure 2.

Figure 2

Effect of high-fructose, high-fat diet (HFF) on intramyocellular lipid (IMCL) content and skeletal muscle fiber type in juvenile Iberian pigs (n = 20). (A) Representative image of Oil red O (ORO) staining of longissimus dorsi muscle fibers [400× total magnification] with percentage of fibers staining with ORO (n = 3646) and ORO stain intensity (n = 918) (arbitrary units (AU)) demonstrating decreased IMCL in HFF-fed compared with CON-fed pigs; (B) representative image of immunohistochemical fiber typing of longissimus dorsi muscle [400× total magnification] with the total percentage of type I, type IIa, and type IIb/x fibers stained (n = 12682) indicating a decrease in type I slow oxidative fibers. Blue stained cells—Type I skeletal muscle fibers; green-stained cells—Type IIa; red-stained cells—Type IIb; unstained cells—Type IIx; CON—control; CON-N—control diet without probiotic supplementation; CON-P—control diet with probiotic supplementation; HFF-N—high-fructose, high-fat diet without probiotic supplementation; HFF—P-high-fructose, high-fat diet with probiotic supplementation. Statistical significance between groups is indicated by * (p ≤ 0.05), *** (p ≤ 0.001). Non-significant differences between groups are indicated by “n.s.’’.