Table 1.

Primer combination and thermal cycling conditions used to quantify the 16S rRNA.

Primer 16S rRNA	Sequences (5′-3′)	Terminal Cycling	Reference
341F	CCTACGGGAGGCAGCAG	(98 °C 3 min); (98 °C 30 s, 55 °C 30 s, 72 °C 40 s) × 25; 72 °C 10 min; 10 °C hold	[34]
805R	GACTACNVGGGTATCTAATCC	(98 °C 3 min); (98 °C 30 s, 55 °C 30 s, 72 °C 45 s) × 8; 72 °C 5 min; 10 °C hold