Figure 5.

Expression characteristics of the PD1 gene. (A) RNA accumulation of the PD1 gene in different tissues and developmental stages. Total RNA was extracted from seven-day-old green seedlings and etiolated seedlings, roots, cotyledons, leaves, stems, flowers, and siliques and underwent formaldehyde electrophoresis and northern blot analyses. 10 μg total RNA per lane. (B) GUS staining of transgenic plants expressing GUS gene driven by PD1 promoter. (a) ten-day-old transgenic seedling expressing GUS gene driven by PD1 promoter. (b) ten-day-old seedling transformed pCAMBIA1381Z empty vector. (c–g) rosette leaf, stem, cauline leaf, flower, and silique from the transgenic plants expressing GUS gene driven by PD1 promoter.