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Accumulation of PEP-β subunit is defective in the pd1-1 mutant. Total leaf protein prepared from wild type and pd1-1 seedlings was separated by SDS–PAGE electrophoresis and analyzed by immunoblot with specific antibodies for PEP-β. Accumulation of PEP-α and PEP-β′ was examined parallelly. Accumulation of Actin was the reference of the loading sample.
