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. 2021 Dec 14;19(12):706. doi: 10.3390/md19120706

Figure 4.

Figure 4

Fluorescent HPLC analyses of the degradation orientation of rAly6, Pae-rAlgL, and Avi-rAlgL. (A) 2-AB-M5 degraded by rAly6; (B) 2-AB-G5 degraded by rAly6; (C) 2-AB-UDP5 degraded by rAly6; (D) 2-AB-M5 degraded by Pae-rAlgL; (E), 2-A B-G5 degraded by Avi-rAlgL. E (−), control treated with inactivated enzymes accordingly. The resulting products were analyzed on a SuperdexTM peptide 30 Increase 10/300 GL gel filtration column monitored using a fluorescent detector with an excitation wavelength of 330 nm and an emission wavelength of 420 nm.