Figure 8.

The intracellular effects of αSynO are diminished in the presence of M30. (A) Postsynaptic current recordings of primary hippocampal neurons were intracellularly recorded with a solution containing 1 μM of αSynO, in the absence and presence of 5 μM M30, for 24 h. Control condition shows the presence of spontaneous synaptic currents (arrows in blue) along with spikes in the current recording mode (arrows in red) and both currents are increased when αSynO 1 µM is applied intracellularly through the recording electrode. Oligomerization in the presence of M30 decreases this effect. (B–D) Quantification of charge transferred (fC), frequency (Hz), and amplitude (pA) from the conditions described in (A). Bars represent the mean ± SEM from the indicated number of neurons (* p < 0.05, ** p < 0.01; *** p < 0.001; one-way Welch’s ANOVA with Dunnett’s T3 post hoc test).